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학술지 Facile Photopatterning of Polyfluorene for Patterned Neuronal Networks
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백남섭, 김용희, 한영환, 이봉준, 김태동, 김신태, 최영석, 김국화, 정명애, 정상돈
Soft Matter, v.7 no.21, pp.10025-10031
Royal Society of Chemistry (RSC)
10SC1500, 인공망막 이식용 생체적합성 나노-바이오 다중전극어레이 칩 기술 개발, 정상돈
In this paper, we demonstrated a facile photopatterning method that uses photocrosslinkable polyfluorene to fabricate micro-sized photopatterns on transparent indium tin oxide substrate for neuronal patterning. The modified poly(ethyleneimine) (m-PEI) with trimethoxysilane moiety was chemically attached to the hydroxyl group-terminated ITO surface and then the photopatternable polyfluorene derivative was spin coated as a cell-repellent layer onto the m-PEI-coated surface. The well-defined micropatterns were easily created over an entire surface by photocrosslinking of bromoalkyl-substituted polyfluorene (Br-PF) via the radical coupling reaction of a C-Br bond under UV irradiation without an initiator. UV-Vis absorbance, photoluminescence, ATR-FTIR and X-ray photoelectron spectroscopy were used to confirm the photocrosslinking process and the surface composition before and after the photocrosslinking of polyfluorene. The pairing of adhesive m-PEI and repulsive Br-PF effectively guided the neurite outgrowth and controlled neurite extension from individual neurons to the pre-patterned direction with excellent pattern fidelity. Guided neuronal cells were maintained for at least 25 days in vitro without any detachment of neuronal cells during cell culture. A photopatternable polyfluorene derivative in combination with cell-adhesive m-PEI is proved to be an effective way to modify the electrode surface to achieve single cell level neuronal networks. © 2011 The Royal Society of Chemistry.