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학술지 Rapid Agglutination of Whole Blood by Antiserum and its Integration in a Blood Pretreatment Module with Dual Function
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저자
최요한, 신중훈, 성건용, 김창범, 정광효
발행일
201503
출처
Sensors and Actuators B : Chemical, v.208, pp.450-456
ISSN
0925-4005
출판사
Elsevier
DOI
https://dx.doi.org/10.1016/j.snb.2014.11.062
협약과제
13SE1500, 특이질병 진단 센싱 어레이 셀칩 및 송수신 기술(총괄과제명 : 생체진단 가능 시각/미각 수용체 기반 센싱 기술 개발), 송기봉
초록
Blood pretreatment for many clinical applications in most situations involves removing blood cells, and today mainly depends on centrifugation. Centrifugation, however, needs machinery which consumes power. In the point-of-care-testing area in particular, harvesting plasma or serum from undiluted whole blood has been both an important hurdle and target at the same time. We describe herein a rapid and integrated method for the separation of plasma from undiluted blood in dozens of seconds without any power supply. We generated polyclonal antibodies from mice which captures human blood cells to make cellular aggregates, resulting in agglutination in a few seconds. The generated anti-human blood antiserum accelerated the sedimentation of blood cells, which was four times faster than the natural sedimentation, even from undiluted whole blood. Additionally, a plasma albumin reduction module was combined to make a pretreatment module having dual functions. The volume of recovered plasma increased with the decrease in albumin. The pretreatment of undiluted whole blood was finished in 30 s without any detectable blood cells using this dual function pretreatment module.
키워드
Agglutination, Albumin, Antibody, Blood pretreatment, Filter
KSP 제안 키워드
Dual function, Human blood cells, Point-of-Care testing, Polyclonal antibodies, clinical application, power supply, whole blood