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학술지 Analysis of Beta Amyloid Peptides Employing the Small Probe Molecules
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저자
홍효봉, Nam In-Hyun, 손승원, 송기봉
발행일
201306
출처
Journal of Biomedical Nanotechnology, v.9 no.6, pp.1088-1091
ISSN
1550-7033
출판사
American Scientific Publisher (ASP)
DOI
https://dx.doi.org/10.1166/jbn.2013.1515
협약과제
11SF1300, 생체진단 가능 시각/미각 수용체 기반 센싱 기술 개발, 송기봉
초록
We herein describe an analytical method employing a small molecule array for the characterization of similar proteins based on ligand binding. In this study, 2 different beta amyloids (A棺(1-40) and (1-42)) were selected as the model compounds. Their primary structures are identical except for 2 additional C-terminal amino acids. However, many studies have observed different biological and chemical characteristics of these peptides. Thus, the ability to distinguish these 2 peptides is important in the diagnosis and development of treatments for related disorders such as Alzheimer's disease. However, strong non-specific binding is usually observed, even when specific antibodies for each peptide are employed. In this study, A棺(1-40) and A棺(1-42) peptides were immobilized on a typical 96-well microplate. Twenty different small probe molecules (modified amino acids conjugated with FITC) were applied to the peptides acting as the secondary antibodies and labeling compounds. The results show that specific binding patterns occurred according to A棺 type and the analysis of the patterns can be used to distinguish these 2 similar peptides. Copyright © 2013 American Scientific Publishers All rights reserved.
키워드
Array, Beta amyloid, Pattern analysis, Small probe molecules
KSP 제안 키워드
96-well, Alzheimer's Disease(AD), Analytical Method, Beta-amyloid, Chemical characteristics, Model compounds, Modified amino acids, Non-specific binding, Specific antibodies, amyloid peptides, ligand binding