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학술지 In Situ Measurement of Superoxide and Hydroxyl Radicals by Frequency Mixing Detection Technique
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저자
홍효봉, Hans Joachim Krause, 손승원, TaiKyong Baik, 박종현, 신성웅, ChiHu Park, DaeYong Song
발행일
201402
출처
Analytical Biochemistry, v.447 no.1, pp.141-145
ISSN
0003-2697
출판사
Elsevier
DOI
https://dx.doi.org/10.1016/j.ab.2013.11.009
협약과제
13VC1100, 실감미디어를 위한 플렉시블 센서 기반 촉감 저장·재생 플랫폼 원천기술 개발, 홍효봉
초록
Frequency mixing magnetic detection (FMMD) was used to detect superoxide from hypoxanthine and xanthine reaction and to detect hydroxyl radical from the Fenton reaction. FMMD was also applied to measure the reactive oxygen species (ROS) level released from microglial cells. We could assess the formation and extinction of the free radicals without a spin trap reagent. The FMMD signal amplitude scaled with the concentration of the radicals. It was verified that no signals are obtained from the substrates and reagents. Based on the observations and on previous research, we suggest that the FMMD signals originate from superoxide and hydroxyl radicals, indicating that FMMD can be used to detect O-centered radicals. Subsequent analysis of free radicals generated from living microglial cells showed that there were significant differences between the activated microglial cells and resting ones. The results of this research are promising regarding the applications of FMMD for in situ measurement of free radicals from various sources, including the cell. © 2013 Elsevier B.V. All rights reserved.
키워드
Analysis, Fenton, FMMD, Hypoxanthine, Microglial cells, Reactive oxygen species, Xanthine
KSP 제안 키워드
Fenton reaction, Hydroxyl radical(OH), Reactive oxygen species (ros), detection techniques, free radicals, frequency mixing, magnetic detection, reactive oxygen species, signal amplitude, situ measurement, spin trap