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학술지 Photo Selective Protein Immobilization Using Bovine Serum Albumin
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저자
김완중, 김안순, 허철, 박찬우, 아칠성, 김봉규, 양종헌, 정광효, 최요한, 홍종철, 성건용
발행일
201211
출처
Applied Surface Science, v.261, pp.880-889
ISSN
0169-4332
출판사
Elsevier Science, Elsevier,
DOI
https://dx.doi.org/10.1016/j.apsusc.2012.08.111
협약과제
12ZC1100, 메가컨버전스 핵심기술 개발, 김채규
초록
A simple and selective technique which immobilizes protein onto a solid substrate by using UV illumination has been developed. In protein immobilization, a Bovine serum albumin (BSA) performed bifunctional role as a cross-linker between substrate and proteins and as a blocker inhibiting a nonspecific protein adsorption. A new photo-induced protein immobilization process has been investigated at each step by fluorescence microscopy, ellipsometry, and Fourier transform infrared (FT-IR) spectroscopy. A UV photomask has been used to induce selective protein immobilization on target regions of the surface of the SiO 2 substrates under UV illumination with negligible nonspecific binding. The UV illumination also showed improved photostability than the conventional methods which employed bifunctional photo-crosslinker molecules of photo-reactive diazirine. This new UV illumination-based photo-addressable protein immobilization provides a new approach for developing novel protein microarrays for multiplexed sensing as well as other types of bio-immobilization in biomedical devices and biotechnologies. © 2012 Elsevier B.V. All rights reserved.
키워드
Bovine serum albumin (BSA), Photo-crosslinker, Protein immobilization
KSP 제안 키워드
And Proteins, Biomedical Devices, Conventional methods, Fluorescence Microscopy, Fourier Transform Infrared Spectroscopy(FTIR), Fourier transform infrared (FT-IR) spectroscopy, Immobilization process, Induced protein, New approach, Non-specific binding, Photo-crosslinker