상세정보
학술지
Selective Binding and Detection of Magnetic Labels Using PHR Sensor via Photoresist Micro-Wells
- 발행일
- 201105
- 출처
- Journal of Nanoscience and Nanotechnology, v.11 no.5, pp.4452-4456
- ISSN
- 1533-4880
- 출판사
- American Scientific Publishers (ASP)
- 협약과제
- 10ZC1100, 메가컨버전스 핵심기술 개발, 함호상
- 초록
- We have developed a novel platform for selective binding of magnetic labels on planar Hall resistance sensor (PHR) for biosensing applications. The photoresist (PR) micro wells were prepared on the PHR sensor junctions to trap the magnetic bead at specified locations on the sensor surface and thin layer of Au was sputtered in the PR wells immobilize bimolecular. The Au surface is functionalized with single-stranded oligonucleotide and further biotin was used to immobilize streptavidin coated magnetic labels (Dynabeads Myone 1.0 μm, Invitrogen Co.). After removal of the PR wells on the sensor surface the non specific binding magnetic labels were successfully removed and only the chemically bounded magnetic labels were remained on the Au surface for detection of biomolecules using PHR sensor. We controlled the number of magnetic labels on the PHR sensor surface by using different sizes of the PR well on the junctions. The specifically bounded magnetic labels were successfully detected by characterizing the individual PHR sensor junctions. This technique enables the complete control over the magnetic labels for selective binding of biomolecules on the sensor surface for increasing the sensitivity of the PHR sensor as well as removal of the non specific bindings on the sensor surface. Copyright © 2011 American Scientific Publishers. All rights reserved.
- 키워드
- Biosensor, Magnetic labels, Micro well, Non specific binding, PHR sensor
- KSP 제안 키워드
- Au surface, Different sizes, Hall resistance, Magnetic beads(MBs), Magnetic labels, Single-stranded, resistance sensor, selective binding, specific binding, thin layer