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학술지 Detection of Uncharged or Feebly Charged Small Molecules by Field-Effect Transistor Biosensors
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저자
아칠성, 박찬우, 양종헌, 이준성, 김완중, 정광효, 최요한, 백인복, 김정호, 성건용
발행일
201203
출처
Biosensors and Bioelectronics, v.33 no.1, pp.233-240
ISSN
0956-5663
출판사
Elsevier,
DOI
https://dx.doi.org/10.1016/j.bios.2012.01.010
협약과제
11MC1600, 프로그램 가능한 바이오 CMOS 전계형 소자, 성건용
초록
This paper describes a new technique for the detection of uncharged or feebly charged small molecules (<400. Da) using Si field-effect transistor (FET) biosensors that are signal-enhanced by gold nanoparticle (NP) charges under dry measurement conditions. NP charges are quickly induced by a chemical deposition (that is, Au deposition) and the indirect competitive immunogold assay, and strongly enhance the electrical signals of the FET biosensors. For the validation of signal enhancement of FET biosensors based on NP charges and detection of uncharged or feebly charged small molecules, mycotoxins (MTXs) of aflatoxin-B1 (AFB1), zearalenone (ZEN), and ochratoxin-A (OTA) were used as target molecules. According to our experimental results, the signal is 100 times more enhanced than the use of the existing solution FET biosensing techniques. Furthermore, this method enables the FET biosensor to quantitatively detect target molecules, regardless of the ionic strengths, isoelectric points (p. I), or pHs of the measured sample solutions. © 2012 Elsevier B.V.
키워드
Field-effect transistor, Mycotoxin, Nanoparticle charge, Signal enhancement, Small molecule detection
KSP 제안 키워드
Au deposition, Chemical deposition, Electrical signal, Field-effect transistors(FETs), Immunogold assay, Isoelectric point(IEP), Nanoparticle charge, Ochratoxin-A, Si field-effect transistor, gold nanoparticles(NPs), signal enhancement