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학술지 Electronic Detection of Biomarkers by Si Field-Effect Transistor from Undiluted Sample Solutions with High Ionic Strengths
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저자
아칠성, 김안순, 김완중, 박찬우, 안창근, 양종헌, 백인복, 김태엽, 성건용
발행일
201006
출처
Bulletin of the Korean Chemical Society, v.31 no.6, pp.1561-1567
ISSN
0253-2964
출판사
대한화학회 (KCS)
DOI
https://dx.doi.org/10.5012/bkcs.2010.31.6.1561
협약과제
09MC3300, 프로그램 가능한 바이오 CMOS 전계형 소자, 성건용
초록
In this study, we have developed a new detection method using Si field effect transistor (FET)-type biosensors, which enables the direct monitoring of antigen-antibody binding within very high-ionic-strength solutions such as lxPBS and human serum. In the new method, as no additional dilution or desalting processes are required, the FET-type biosensors can be more suitable for ultrasensitive and real-time analysis of raw sample solutions. The new detection scheme is based on the observation that the strength of antigen-antibody- specific binding is significantly influenced by the ionic strength of the reaction solutions. For a prostate specific antigen (PSA), in some conditions, the binding reaction between PSA and anti-PSA in a low-ionic strength reaction solution such as 10 μM phosphate buffer is weak (reversible), while that in high-ionic strength reaction solutions such as lxPBS or human serum is strong.
키워드
Electronic detection, Ionic strength, PSA, Real-time, Si field effect transistor (FET)
KSP 제안 키워드
Antigen-antibody binding, Binding reaction, Detection Method, Detection scheme, Electronic detection, Field-effect transistors(FETs), Human serum, Ionic strength, Phosphate buffer, Real-time Analysis, Si field-effect transistor