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학술지 Detection of Two Different Influenza A Viruses Using a Nitrocellulose Membrane and a Magnetic Biosensor
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저자
홍효봉, Hans-Joachim Krause, 송기봉, Chel-Jong Choi, 정명애, 손승원, Andreas Offenhausser
발행일
201102
출처
Journal of Immunological Methods, v.365 no.1-2, pp.95-100
ISSN
0022-1759
출판사
Elsevier
DOI
https://dx.doi.org/10.1016/j.jim.2010.12.005
협약과제
09SC1100, 생체진단 가능 시각/미각 수용체 기반 센싱 기술, 송기봉
초록
Here we describe a new analytical method for the detection of two influenza A viruses by nitrocellulose membrane and magnetic sensors that employ a special frequency mixing technique. The combination of the nitrocellulose membrane and magnetic bead detection permits a rapid assay procedure and excludes two steps (the development of color and the stop reaction) required for usual immunochemical detection methods such as ELISA. Quantitative virus detection was performed using magnetic beads conjugated with secondary antibody. The results were compared with conventional assay methods and with a dot-blot assay with fluorescence compound (FITC). Under optimum conditions, our new assay procedure is capable of detecting picograms of virus per well. This new method combining the nitrocellulose membrane and magnetic bead detection reduces analytical time and allows stable and repeatable analyses of samples in point-of-care applications. © 2010 Elsevier B.V.
키워드
Immunodetection, Immunoflow assay, Influenza A virus, Magnetic assay
KSP 제안 키워드
Analytical Method, Assay methods, Detection Method, Fluorescence compound, Influenza A viruses, Magnetic beads(MBs), Magnetic biosensor, Magnetic sensor, Mixing technique, Optimum condition, Point-of-care