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학술지 A Temperature-Controllable Microelectrode and Its Application to Protein Immobilization
Cited 8 time in scopus Download 1 time Share share facebook twitter linkedin kakaostory
저자
이대식, 최형길, 정광효, 이번열, 표현봉, 윤현철
발행일
200710
출처
ETRI Journal, v.29 no.5, pp.667-669
ISSN
1225-6463
출판사
한국전자통신연구원 (ETRI)
DOI
https://dx.doi.org/10.4218/etrij.07.0207.0035
협약과제
06MB3200, 유비쿼터스 건강관리용 모듈 시스템, 박선희
초록
This letter presents a smart integrated microfluidic device which can be applied to actively immobilize proteins on demand The active component in the device is a temperature-controllable microelectrode array with a smart polymer film, poly(N-isopropylacrylamide) (PNIPAAm) which can be thermally switched between hydrophilic and hydrophobic states. It is integrated into a micro hot diaphragm having an integrated micro heater and temperature sensors on a 2-micrometer-thick silicon oxide/silicon nitride/silicon oxide (O/N/O) template. Only 36 mW is required to heat the large template area of 2 mm×16 mm to 40°C within 1 second. To relay the stimulus-response activity to the microelectrode surface, the interface is modified with a smart polymer. For a model biomolecular affinity test, an anti-6-(2, 4-dinitrophenyl) aminohexanoic acid (DNP) antibody protein immobilization on the microelectrodes is demonstrated by fluorescence patterns.
키워드
BioMEMS sensor, Immobilization, Micro hot plate, Microelectrode, Smart polymer
KSP 제안 키워드
2 mm, Active components, Micro-heater, Micro-hotplate(MHP), Microfluidic device, N-isopropylacrylamide(NIPAm), Polymer films, Silicon Nitride, Silicon oxide, Stimulus-response, Thick silicon