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학술지 Azimuthal Phase Retardation Microscope for Visualizing Actin Filaments of Biological Cells
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저자
신인희, 신상모
발행일
201109
출처
Journal of Biomedical Optics, v.16 no.9, pp.1-5
ISSN
1083-3668
출판사
SPIE
DOI
https://dx.doi.org/10.1117/1.3615665
협약과제
11ZV1200, 산업계 기술지원사업, 김영선
초록
We developed a new theory-based azimuthal phase retardation microscope to visualize distributions of actin filaments in biological cells without having them with exogenous dyes, fluorescence labels, or stains. The azimuthal phase retardation microscope visualizes distributions of actin filaments by measuring the intensity variations of each pixel of a charge coupled device camera while rotating a single linear polarizer. Azimuthal phase retardation {\\lambda} between two fixed principal axes was obtained by calculating the rotation angles of the polarizer at the intensity minima from the acquired intensity data. We have acquired azimuthal phase retardation distributions of human breast cancer cell, MDA MB 231 by our microscope and compared the azimuthal phase retardation distributions with the fluorescence image of actin filaments by the commercial fluorescence microscope. Also, we have observed movement of human umbilical cord blood derived mesenchymal stem cells by measuring azimuthal phase retardation distributions. © 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).
키워드
imaging systems, microscopes, microscopy
KSP 제안 키워드
Actin filaments(AF), Biological cells, Breast Cancer(BC), Charge coupled device, Fluorescence Microscope, Human breast cancer, Intensity variation, Linear polarizer, Optical instrumentation, Principal axis, Umbilical cord blood