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학술지 Synthesis of Polystyrene Nanoparticles with Different Surface Modification by Emulsion Polymerization and Measurement of IgG Adsorption and Stability for the Application in Latex-Protein Complex Based Solid-Phase Immunoassay
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저자
김상희, 김철암, 최요한, 정문연
발행일
200901
출처
Polymer Bulletin, v.62 no.1, pp.23-32
ISSN
0170-0839
출판사
Springer
DOI
https://dx.doi.org/10.1007/s00289-008-1010-y
협약과제
08MC2500, 유비쿼터스 건강관리용 모듈 시스템, 박선희
초록
Unmodified and surface modified polystyrene (PS) nanoparticles with hydrophilic end group on surface were synthesized by emulsion polymerization for adsorption of a protein (immunoglobulin G, IgG) by passive adsorption and electrostatic attraction for the application on solid-phase immunoassay. The presence of formed IgG-PS particle complex was confirmed by dynamic light scattering (DLS) measurement which shows the increased size of particle (≈178nm and 220nm) after IgG immobilization. Increased Zeta (瓘) potential from -56.7mV to -3.84mV following the IgG immobilization by electrostatic attraction also provides the presence of the formed IgG-PS particle complex. Enzyme-linked immunosorbent assay (ELISA) results demonstrate that the formed IgG-PS complex was stable and the binding strength between IgG and PS was maintained after 1 month from initial IgG-PS particle complex formation. © 2008 Springer-Verlag.