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학술지 Some Key Factors in a Bead-based Fluorescence Immunoassay
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저자
김용준, 김혜윤, 아칠성, 정문연, 박선희
발행일
200803
출처
BioChip Journal, v.2 no.1, pp.60-65
ISSN
1976-0280
출판사
한국바이오칩학회
협약과제
08MC2500, 유비쿼터스 건강관리용 모듈 시스템, 박선희
초록
In an effort to understand some key factors in a bead-based fluorescence immunoassay, a series of sandwich-type immunoassays were carried out on a glass plate using a latex bead embedded with dye molecules. The fluorescence bead functionalized with an amine functionality was conjugated with an anti- prostate specific antigen (anti-PSA) using glutaraldehyde. The glass surface was functionalized with the amine group using 3-aminopropltriethoxysilane(3-APT). The amine functionality on the glass surface was converted to aldehyde usinglutaraldehyde followed by conjugation with the anti-PSA. A series of sandwich-type immunoassays were carried out by incubating the anti-PSA bead with PSA followed by incubating the PSA/bead complex on the anti-PSA glass surface. The dependency of the fluorescence intensity in the immunoassay was observed to increase when the fluorescence bead concentration was increased from 10-3 mg/mL to 101 mg/mL with some signs of saturation around the range of 100 mg/mL to 101 mg/mL. Also, the fluorescence intensity was observed toincrease when the incubation time of the PSA /anti-PSA bead complex on the anti-PSA glass was increased from 5 minutes to 60minutes. The shear force generated during the washing step was observed to affect the bead-based immunoassay.
KSP 제안 키워드
Amine group, Fluorescence intensity, Glass plate, Glass surface, Incubation time, Key factor, Latex bead, amine functionality, bead-based immunoassay, dye molecules, fluorescence immunoassay