BROWSE
Detail
Conference Paper
Immunodetection of Cancer Marker in Human Serum using Silicon Field Effect Transistors
Cited - time in 
Share 
Share
- Authors
- An Soon Kim, Chil Seong Ah, Chan Woo Park, Jong Heon Yang, Tae Youb Kim, Chang Guen Ahn, Gun Yong Sung
- Issue Date
- 2009-05
- Citation
- Nanotech conference and Expo 2009, pp.1-2
- Language
- English
- Type
- Conference Paper
- Abstract
- Label-free and real-time carcinoembryonic antigen (CEA) sensor has been developed using p-type silicon field-effect transistor (FET) where the conventional "top-down" semiconductor processes were employed to make nanostructures. We have detected the CEA as a marker for colorectal cancer 1 in undiluted human serum with high salt concentration (~150 mM) using the Si-FET. To detect the CEA, the monoclonal antibody of CEA (anti-CEA) has been immobilized on the Si surface through covalent linkage using specific surface chemistry. Specific binding of CEA with the anti-CEA on p-type Si-FET channels leads to a conductivity change in response to variations of electric field at the surface, which results in label-free and real-time immunodetection. It has been known that detecting a target antigen in human serum is difficult because of short Debye screening length of the serum with high salt concentration. 2 In this presentation, we discuss new detection method of the target antigen, CEA, in human serum and show quantitative detection results of CEA in human serum from 1 ng/mL to 100 ng/mL using the Si-FET sensor.
- KSP Keywords
- Cancer marker, Carcinoembryonic antigen, Colorectal Cancer, Conductivity change, Covalent linkage, Debye screening length, Detection Method, Electric Field, FET sensor, Field Effect Transistor(FET), High salt