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학술지 Analysis of Configuration of Surface-Immobilized Proteins by Si Nanochannel Field Effect Transistor Biosensor
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저자
박찬우, 아칠성, 안창근, 양종헌, 김안순, 김태엽, 성건용
발행일
201106
출처
Sensors and Actuators B : Chemical, v.154 no.2, pp.164-168
ISSN
0925-4005
출판사
Elsevier
DOI
https://dx.doi.org/10.1016/j.snb.2009.11.056
협약과제
09MC3300, 프로그램 가능한 바이오 CMOS 전계형 소자, 성건용
초록
We demonstrate the application of 'top-down' fabricated Si nanochannel field effect transistor biosensors for measuring charging properties or configuration of proteins immobilized on the Si surface. We have immobilized monoclonal antibodies for prostate specific antigen (anti-PSA) on the p-type Si channel surface, and monitored the channel conductance in response to the variation of ionic strength (salt concentration) and pH of the buffer solution. Effects of the salt concentration at a constant pH is consistent with the screening of protein charges by dissolved counter-ions, enabling analysis of the net protein charge and spatial configuration based on calculated values of Debye screening length ({\\lambda}D). The pH-dependence of channel conductance at varying salt concentration has also been investigated, from which the relationship between the pH and net protein charge can be estimated. © 2009 Elsevier B.V.
키워드
Biosensor, Field effect transistor, Protein, Si nanochannel
KSP 제안 키워드
Buffer solution, Constant pH, Counter-ions, Debye screening length, Field effect transistor biosensor, Field-effect transistors(FETs), Ionic strength, Monoclonal antibody(MAb), Salt concentration, Si channel, Si nanochannel