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학술지 Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays
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저자
김완중, 조효영, 정봉진, 변상원, 허재두, 김용준
발행일
201801
출처
Sensors, v.18 no.1, pp.1-12
ISSN
1424-8220
출판사
MDPI AG
DOI
https://dx.doi.org/10.3390/s18010055
협약과제
17JS1700, 스마트폰연계 타액 중 다중 바이오마커 기반 스트레스 분석기, 김용준
초록
Using gold nanoparticles (AuNPs) on ?쐁apillary enzyme-linked immunosorbent assay (ELISA)", we produced highly sensitive and rapid assays, which are the major attributes for point-of-care applications. First, in order to understand the size effect of AuNPs, AuNPs of varying diameters (5 nm, 10 nm, 15 nm, 20 nm, 30 nm, and 50 nm) conjugated with Horseradish Peroxidase (HRP)-labeled anti-C reactive protein (antiCRP) (AuNP?줭ntiCRP-HRP) were used for well-plate ELISA. AuNP of 10 nm produced the largest optical density, enabling detection of 0.1 ng/mL of CRP with only 30 s of incubation, in contrast to 10 ng/mL for the ELISA run in the absence of AuNP. Then, AuNP of 10 nm conjugated with antiCRP-HRP (AuNP?줭ntiCRP-HRP) was used for ?쐁apillary ELISA?? to detect as low as 0.1 ng/mL of CRP. Also, kinetic study on both 96-well plates and in a capillary tube using antiCRP-HRP or AuNP?줭ntiCRP-HRP showed a synergistic effect between AuNP and the capillary system, in which the fastest assay was observed from the ?쏛uNP capillary ELISA??, with its maximum absorbance reaching 2.5 min, while the slowest was the typical well-plate ELISA with its maximum absorbance reaching in 13.5 min.
키워드
C-reactive protein (CRP), Capillary ELISA, Immunogold conjugate, Point-of-care (POC)
KSP 제안 키워드
20 nm, 5 nm, 96-well, C-reactive protein(CRP), Capillary system, Capillary tube, Enzyme-linked immunosorbent assay, High Sensitivity, Highly sensitive, Kinetic study, Optical density
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