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학술지 The Protective Effects of EMF-LTE against DNA Double-Strand Break Damage In Vitro and In Vivo
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진희, 김규리, 박가영, 김민정, 이해준, 전상봉, 김주환, 김학림, 임경민, 이윤실
International Journal of Molecular Sciences, v.22 no.10, pp.1-14
21HH2400, 복합 전파환경에서의 국민건강 보호 기반 구축 사업, 최형도
With the rapid growth of the wireless communication industry, humans are extensively exposed to electromagnetic fields (EMF) comprised of radiofrequency (RF). The skin is considered the primary target of EMFs given its outermost location. Recent evidence suggests that extremely low frequency (ELF)?륟MF can improve the efficacy of DNA repair in human cell?릐ines. However, the effects of EMF?륱F on DNA damage remain unknown. Here, we investigated the impact of EMF-long term evolution (LTE, 1.762 GHz, 8 W/kg) irradiation on DNA double?릗trand break (DSB) using the murine melanoma cell line B16 and the human keratinocyte cell line HaCaT. EMF?륧TE exposure alone did not affect cell viability or induce apoptosis or necrosis. In addition, DNA DSB damage, as determined by the neutral comet assay, was not induced by EMF?륧TE irradiation. Of note, EMF?? LTE exposure can attenuate the DNA DSB damage induced by physical and chemical DNA dam-aging agents (such as ionizing radiation (IR, 10 Gy) in HaCaT and B16 cells and bleomycin (BLM, 3 μM) in HaCaT cells and a human melanoma cell line MNT??1), suggesting that EMF?륧TE promotes the repair of DNA DSB damage. The protective effect of EMF?륧TE against DNA damage was further confirmed by attenuation of the DNA damage marker 款?륣2AX after exposure to EMF?륧TE in Ha?? CaT and B16 cells. Most importantly, irradiation of EMF?륧TE (1.76 GHz, 6 W/kg, 8 h/day) on mice in vivo for 4 weeks reduced the 款?륣2AX level in the skin tissue, further supporting the protective effects of EMF?륧TE against DNA DSB damage. Furthermore, p53, the master tumor?릗uppressor gene, was commonly upregulated by EMF?륧TE irradiation in B16 and HaCaT cells. This finding suggests that p53 plays a role in the protective effect of EMF?륧TE against DNA DSBs. Collectively, these results demonstrated that EMF?륧TE might have a protective effect against DNA DSB damage in the skin, although further studies are necessary to understand its impact on human health.
B16, DNA damage, Electromagnetic waves, HaCaT, LTE, Repair gene expression
KSP 제안 키워드
6 GHz, Cell line, Cell viability, Communication industry, DNA Damage, DNA double-strand break, DNA repair, Electromagnetic Field, Electromagnetic waves(EM), Extremely Low Frequency, Gene expression
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