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Direct Detection System for Escherichia Coli Using Au-Ag Alloy Microchips
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- Authors
- Wan-Joong Kim, Sanghee Kim, Ae Rhan Kim, Dong Jin Yoo
- Issue Date
- 2013-06
- Citation
- Industrial & Engineering Chemistry Research, v.52, no.22, pp.7282-7288
- ISSN
- 0888-5885
- Publisher
- American Chemical Society(ACS)
- Language
- English
- Type
- Journal Article
- Abstract
- Au-Ag alloy nanoparticles (NPs) were prepared by the reduction of metal ion mixtures in aqueous sodium citrate solution using sodium borohydride (NaBH 4). The resulting Au-Ag alloy NPs were analyzed by various techniques. Alloy-attached chips for the detection of microorganisms were fabricated simply by the attachment of Au-Ag alloy nanoparticles onto glass slides after silanization through self-assembled monolayers (SAMs) for the formation of activated amine (-NH2) as a terminal function group. The alloy-attached chips were investigated for their ability to bind the target Escherichia coli (E. coli) in water. E. coli was detected in water as a function of time and concentration by UV-vis spectroscopic measurements based on the interaction between the alloy-attached chip and E. coli. Field-emission scanning electron microscopy (FE-SEM) was used to directly observe the E. coli captured on the alloy chips. These studies demonstrated that E. coli in drinking water can be directly detected with Au-Ag alloy microchips without requiring any interaction between an antibody and an antigen. © 2013 American Chemical Society.
- KSP Keywords
- Au-Ag alloy nanoparticles, Detection Systems(IDS), Drinking water, Escherichia coli(E. coli), Field-emission scanning electron microscopy(FE-SEM), Function group, Glass slides, Metal ion, NaBH 4, Scanning electron microscopy(S.E.M.), Self-assembled monolayers(SAMS)