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The Antibody-based Magnetic Microparticle Immunoassay using p-FET Sensing Platform for Alzheimer's Disease Pathogenic Factor
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- Authors
- Chang-Beom Kim, Kwan-Soo Kim, Ki-Bong Song
- Issue Date
- 2013-02
- Citation
- Nano-Bio Sensing, Imaging, and Spectroscopy 2013 (SPIE 8879), v.8879, pp.1-4
- Publisher
- SPIE
- Language
- English
- Type
- Conference Paper
- Abstract
- The importance of early Alzheimer's disease (AD) detection has been recognized to diagnose people at high risk of AD. The existence of intra/extracellular beta-amyloid (A棺) of brain neurons has been regarded as the most archetypal hallmark of AD. The existing computed-image-based neuroimaging tools have limitations on accurate quantification of nanoscale A棺 peptides due to optical diffraction during imaging processes. Therefore, we propose a new method that is capable of evaluating a small amount of A棺 peptides by using photo-sensitive field-effect transistor (p-FET) integrated with magnetic force-based microbead collecting platform and selenium(Se) layer (thickness ~700 nm) as an optical filter. This method demonstrates a facile approach for the analysis of A棺 quantification using magnetic force and magnetic silica microparticles (diameter 0.2~0.3 μm). The microbead collecting platform mainly consists of the p-FET sensing array and the magnet (diameter ~1 mm) which are placed beneath each sensing region of the p-FET, which enables the assembly of the A棺 antibody conjugated microbeads, captures the A棺 peptides from samples, measures the photocurrents generated by the Q-dot tagged with A棺 peptides, and consequently results in the effective A棺 quantification. © 2013 SPIE.
- KSP Keywords
- Alzheimer's Disease(AD), Antibody-based, Beta-amyloid, FET sensing, Field Effect Transistor(FET), Force-based, High risk, Image-based, Magnetic Force, Magnetic microparticle, Magnetic silica