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Conference Paper
Convection-Based Realtime Polymerase Chain Reaction (PCR) Utilizing Transparent Graphene Heaters
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- Authors
- Kwang Hyo Chung, Yo Han Choi, Hong Kyw Choi, Jin Tae Kim, Young-Jun Yu, Jin Sik Choi, Doo-Hyeb Youn, Choon-Gi Choi
- Issue Date
- 2014-11
- Citation
- SENSORS 2014, pp.1-4
- Language
- English
- Type
- Conference Paper
- Abstract
- We present a polymerase chain reaction (PCR) chip incorporating graphene heaters and thermal convection. We adopted graphene heaters in order to replace the bulky metal heating blocks or opaque metal line heaters utilized in the previous PCR chips. Transparency of the CVD based graphene heaters allowed the realtime fluorescence detection of PCR product using a light-path passing through a reaction chamber. Thermal convection enabled rapid and automated PCR thermocycling.
- KSP Keywords
- Metal line, PCR product, Polymerase chain reaction, Reaction chamber, fluorescence detection, thermal convection