상세정보
학술지
Photo Selective Protein Immobilization Using Bovine Serum Albumin
- 발행일
- 201211
- 출처
- Applied Surface Science, v.261, pp.880-889
- ISSN
- 0169-4332
- 출판사
- Elsevier Science, Elsevier,
- 협약과제
- 12ZC1100, 메가컨버전스 핵심기술 개발, 김채규
- 초록
- A simple and selective technique which immobilizes protein onto a solid substrate by using UV illumination has been developed. In protein immobilization, a Bovine serum albumin (BSA) performed bifunctional role as a cross-linker between substrate and proteins and as a blocker inhibiting a nonspecific protein adsorption. A new photo-induced protein immobilization process has been investigated at each step by fluorescence microscopy, ellipsometry, and Fourier transform infrared (FT-IR) spectroscopy. A UV photomask has been used to induce selective protein immobilization on target regions of the surface of the SiO 2 substrates under UV illumination with negligible nonspecific binding. The UV illumination also showed improved photostability than the conventional methods which employed bifunctional photo-crosslinker molecules of photo-reactive diazirine. This new UV illumination-based photo-addressable protein immobilization provides a new approach for developing novel protein microarrays for multiplexed sensing as well as other types of bio-immobilization in biomedical devices and biotechnologies. © 2012 Elsevier B.V. All rights reserved.
- KSP 제안 키워드
- And Proteins, Biomedical Devices, Conventional methods, Fluorescence Microscopy, Fourier Transform Infrared Spectroscopy(FTIR), Fourier transform infrared (FT-IR) spectroscopy, Immobilization process, Induced protein, New approach, Non-specific binding, Photo-crosslinker