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Journal Article
Photo Selective Protein Immobilization Using Bovine Serum Albumin
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- Authors
- Wan-Joong Kim, Ansoon Kim, Chul Huh, Chan Woo Park, Chil Seong Ah, Bong Kyu Kim, Jong-Heon Yang, Kwang Hyo Chung, Yo Han Choi, Jongcheol Hong, Gun Yong Sung
- Issue Date
- 2012-11
- Citation
- Applied Surface Science, v.261, pp.880-889
- ISSN
- 0169-4332
- Publisher
- Elsevier Science, Elsevier,
- Language
- English
- Type
- Journal Article
- Abstract
- A simple and selective technique which immobilizes protein onto a solid substrate by using UV illumination has been developed. In protein immobilization, a Bovine serum albumin (BSA) performed bifunctional role as a cross-linker between substrate and proteins and as a blocker inhibiting a nonspecific protein adsorption. A new photo-induced protein immobilization process has been investigated at each step by fluorescence microscopy, ellipsometry, and Fourier transform infrared (FT-IR) spectroscopy. A UV photomask has been used to induce selective protein immobilization on target regions of the surface of the SiO 2 substrates under UV illumination with negligible nonspecific binding. The UV illumination also showed improved photostability than the conventional methods which employed bifunctional photo-crosslinker molecules of photo-reactive diazirine. This new UV illumination-based photo-addressable protein immobilization provides a new approach for developing novel protein microarrays for multiplexed sensing as well as other types of bio-immobilization in biomedical devices and biotechnologies. © 2012 Elsevier B.V. All rights reserved.
- KSP Keywords
- And Proteins, Conventional methods, Fluorescence microscopy, Fourier transform(FT), Fourier transform infrared (FTIR) spectroscopy, Frustrated total internal reflection(FTIR), Immobilization process, Induced protein, New approach, Non-specific binding, Photo-crosslinker