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학술지 Response to Cardiac Markers in Human Serum Analyzed by Guided-Mode Resonance Biosensor
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김완중, 김봉규, Ansoon Kim, 허철, 아칠성, 김경현, 홍종철, Seon Hee Park, Sanghoon Song, Junghan Song, 성건용
Analytical Chemistry, v.82 no.23, pp.9686-9693
American Chemical Society(ACS)
Cardiac markers in human serum with concentrations less than 0.1 ng/mL were analyzed by use of a guided-mode resonance (GMR) biosensor. Cardiac troponin I (cTnI), creatine kinase MB (CK-MB), and myoglobin (MYO) were monitored in the serum of both patients and healthy controls. Dose - response curves ranging from 0.05 to 10 ng/mL for cTnI, from 0.1 to 10 ng/mL for CK-MB, and from 0.03 to 1.7 μg/mL for MYO were obtained. The limits of detection (LOD) for cTnI, CK-MB, and MYO were less than 0.05, 0.1, and 35 ng/mL, respectively. Analysis time was 30 min, which is short enough to meet clinical requirements. Antibody immobilization and the hydrophilic properties of the guided-mode resonance filter (GMRF) surface were investigated by X-ray photoelectron spectroscopy (XPS) and by monitoring the peak wavelength shift and water contact angle (CA). Both assays used to evaluate the surface density of the immobilized antibodies, a sandwich enzyme-linked immunosorbent assay (ELISA) and a sandwich immunogold assay, showed that the antibodies were successfully immobilized and sufficiently aligned to detect the low concentration of biomarkers. Our results show that the GMR biosensor will be very useful in developing low-cost portable biosensors that can screen for cardiac diseases. © 2010 American Chemical Society.
KSP 제안 키워드
CK-MB, Cardiac Diseases, Cardiac markers, Contact angle(CA), Creatine kinase mb, GMR biosensor, Guided mode resonance filter(GMRF), Guided-mode resonance(GMR), Healthy controls, Human serum, Hydrophilic properties