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Detection of Uncharged or Feebly Charged Small Molecules by Field-Effect Transistor Biosensors
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- Authors
- Chil Seong Ah, Chan Woo Park, Jong-Heon Yang, Joon Sung Lee, Wan-Joong Kim, Kwang Hyo Chung, Yo Han Choi, In Bok Baek, Jungho Kim, Gun Yong Sung
- Issue Date
- 2012-03
- Citation
- Biosensors and Bioelectronics, v.33, no.1, pp.233-240
- ISSN
- 0956-5663
- Publisher
- Elsevier,
- Language
- English
- Type
- Journal Article
- Abstract
- This paper describes a new technique for the detection of uncharged or feebly charged small molecules (<400. Da) using Si field-effect transistor (FET) biosensors that are signal-enhanced by gold nanoparticle (NP) charges under dry measurement conditions. NP charges are quickly induced by a chemical deposition (that is, Au deposition) and the indirect competitive immunogold assay, and strongly enhance the electrical signals of the FET biosensors. For the validation of signal enhancement of FET biosensors based on NP charges and detection of uncharged or feebly charged small molecules, mycotoxins (MTXs) of aflatoxin-B1 (AFB1), zearalenone (ZEN), and ochratoxin-A (OTA) were used as target molecules. According to our experimental results, the signal is 100 times more enhanced than the use of the existing solution FET biosensing techniques. Furthermore, this method enables the FET biosensor to quantitatively detect target molecules, regardless of the ionic strengths, isoelectric points (p. I), or pHs of the measured sample solutions. © 2012 Elsevier B.V.
- KSP Keywords
- Au deposition, Chemical deposition, Electrical signal, Field-effect transistors(FETs), Immunogold assay, Isoelectric point(IEP), Ochratoxin-A, Si field-effect transistor, gold nanoparticles(NPs), signal enhancement, small molecules