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Electronic Detection of Biomarkers by Si Field-Effect Transistor from Undiluted Sample Solutions with High Ionic Strengths
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- Authors
- Chil Seong Ah, Ansoon Kim, Wan-Joong Kim, Chan Woo Park, Chang-Geun Ahn, Jong-Heon Yang, In Bok Baek, Tae-Youb Kim, Gun Yong Sung
- Issue Date
- 2010-06
- Citation
- Bulletin of the Korean Chemical Society, v.31, no.6, pp.1561-1567
- ISSN
- 0253-2964
- Publisher
- 대한화학회 (KCS)
- Language
- English
- Type
- Journal Article
- Abstract
- In this study, we have developed a new detection method using Si field effect transistor (FET)-type biosensors, which enables the direct monitoring of antigen-antibody binding within very high-ionic-strength solutions such as lxPBS and human serum. In the new method, as no additional dilution or desalting processes are required, the FET-type biosensors can be more suitable for ultrasensitive and real-time analysis of raw sample solutions. The new detection scheme is based on the observation that the strength of antigen-antibody- specific binding is significantly influenced by the ionic strength of the reaction solutions. For a prostate specific antigen (PSA), in some conditions, the binding reaction between PSA and anti-PSA in a low-ionic strength reaction solution such as 10 μM phosphate buffer is weak (reversible), while that in high-ionic strength reaction solutions such as lxPBS or human serum is strong.
- KSP Keywords
- Antigen-antibody binding, Binding reaction, Detection Method, Detection scheme, Electronic detection, Field-effect transistors(FETs), Human serum, Ionic strength, Phosphate buffer, Real-time Analysis, Si field-effect transistor