상세정보
학술지
Sensitive “Capillary ELISA” Via Vapor-phase Surface Modification
- 발행일
- 201610
- 출처
- Sensors and Actuators B : Chemical, v.233, pp.281-288
- ISSN
- 0925-4005
- 출판사
- Elsevier
- 협약과제
- 15MC4900, 스마트폰연계 타액 중 다중 바이오마커 기반 스트레스 분석기, 김용준
- 초록
- Sensitive ELISA for C-reactive protein (CRP) was performed in a capillary tube based on simple vapor-phase surface chemistry. Amine surfaces were produced in capillary tubes in two different methods using 3-aminopropyltriethoxysilane (APTES) in liquid and vapor phases. Those amine surfaces were tethered with gold nanoparticles (AuNPs) to be characterized by field emission scanning electron microscopy (FE-SEM) resulting in more dense and dispersed AuNP populations from the vapor-phase surface. Feasibility of a miniaturized home-made optical detector for "capillary ELISA" was performed by infusing into capillary tubes the colored solutions that had just gone through ELISA on a 96-well plate. The compatibility of the two results obtained from ELISAs on a well-plate and in capillary tubes, confirmed dependability of the miniaturized detector. Finally "capillary ELISA" was performed on the home-made detector using anti-CRP immobilized capillary tubes that had been prepared via vapor-phase surface treatment, resulting in detectability of 1.0 ng/mL of CRP, which turned out to be around 10 times higher in sensitivity than that of the well-plate ELISA.
- KSP 제안 키워드
- 3-Aminopropyltriethoxysilane(APTES), 96-well plate, Amine surfaces, C-reactive protein(CRP), Capillary tube, Different methods, Liquid and vapor phases, Optical detector, Scanning electron microscopy(S.E.M.), Surface chemistry, Surface treatments