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Sensitive “Capillary ELISA” Via Vapor-phase Surface Modification
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- Authors
- Wan-Joong Kim, Seok Hwan Hyun, Hyo Young Cho, Sangwon Byun, Bong Kyu Kim, Chul Huh, Kwang Hyo Chung, Young Jun Kim
- Issue Date
- 2016-10
- Citation
- Sensors and Actuators B : Chemical, v.233, pp.281-288
- ISSN
- 0925-4005
- Publisher
- Elsevier
- Language
- English
- Type
- Journal Article
- Abstract
- Sensitive ELISA for C-reactive protein (CRP) was performed in a capillary tube based on simple vapor-phase surface chemistry. Amine surfaces were produced in capillary tubes in two different methods using 3-aminopropyltriethoxysilane (APTES) in liquid and vapor phases. Those amine surfaces were tethered with gold nanoparticles (AuNPs) to be characterized by field emission scanning electron microscopy (FE-SEM) resulting in more dense and dispersed AuNP populations from the vapor-phase surface. Feasibility of a miniaturized home-made optical detector for "capillary ELISA" was performed by infusing into capillary tubes the colored solutions that had just gone through ELISA on a 96-well plate. The compatibility of the two results obtained from ELISAs on a well-plate and in capillary tubes, confirmed dependability of the miniaturized detector. Finally "capillary ELISA" was performed on the home-made detector using anti-CRP immobilized capillary tubes that had been prepared via vapor-phase surface treatment, resulting in detectability of 1.0 ng/mL of CRP, which turned out to be around 10 times higher in sensitivity than that of the well-plate ELISA.
- KSP Keywords
- 3-Aminopropyltriethoxysilane, 96-well plate, Amine surfaces, C-reactive protein(CRP), Capillary tube, Different methods, Field-emission scanning electron microscopy(FE-SEM), Gold Nanoparticles(GNPs), Liquid and vapor phases, Optical detector, Scanning electron microscopy(S.E.M.)