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학술지 Spatial Distribution of Cell-Cell and Cell-ECM Adhesions Regulates Force Balance while Maintaining E-cadherin Molecular Tension in Cell Pairs
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심주용, Jens Moeller, Kevin C. Hart, Diego Ramallo, Viola Vogel, Alex R. Dunn, W. James Nelson, Beth L. Pruitt
Molecular Biology of the Cell, v.26 no.13, pp.2456-2465
American Society for Cell Biology
Mechanical linkage between cell-cell and cell-extracellular matrix (ECM) adhesions regulates cell shape changes during embryonic development and tissue homoeostasis. We examined how the force balance between cell-cell and cell-ECM adhesions changes with cell spread area and aspect ratio in pairs of MDCK cells. We used ECM micropatterning to drive different cytoskeleton strain energy states and cell-generated traction forces and used a F철rster resonance energy transfer tension biosensor to ask whether changes in forces across cell-cell junctions correlated with E-cadherin molecular tension. We found that continuous peripheral ECM adhesions resulted in increased cell-cell and cell-ECM forces with increasing spread area. In contrast, confining ECM adhesions to the distal ends of cell-cell pairs resulted in shorter junction lengths and constant cell-cell forces. Of interest, each cell within a cell pair generated higher strain energies than isolated single cells of the same spread area. Surprisingly, E-cadherin molecular tension remained constant regardless of changes in cell-cell forces and was evenly distributed along cell-cell junctions independent of cell spread area and total traction forces. Taken together, our results showed that cell pairs maintained constant E-cadherin molecular tension and regulated total forces relative to cell spread area and shape but independently of total focal adhesion area.
KSP 제안 키워드
Cell pairs, Cell-cell junctions, E-Cadherin, Embryonic development, Extracellular matrix(ECM), Focal adhesion, Force balance, MDCK cells, Resonance energy transfer, Single cell, Spatial distribution