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Nanogap-Based Electrical PNA Chips for the Detection of Genetic Polymorphism of Cytochrome P450 2C19
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- Authors
- Dae Keun Park, Hyung Ju Park, Cho Yeon Lee, Daewha Hong, Young Lee, Insung S.Choi, Wan Soo Yun
- Issue Date
- 2012-08
- Citation
- Journal of Nanoscience and Nanotechnology, v.12, no.7, pp.5155-5159
- ISSN
- 1533-4880
- Publisher
- American Scientific Publishers (ASP),
- Language
- English
- Type
- Journal Article
- Abstract
- PNA chips for the detection of the genetic polymorphism of Cytochrome P450 2C19 (CYP2C19), a well-known enzyme related to the metabolism of therapeutic drugs, were electrically-interfaced with interdigitated nanogap electrodes (INEs). The average gap distance and effective length of the INEs were about ~70 nm and ~140 μm, respectively. Those INEs having the aspect ratio of about 2000, were prepared by the combination of the photolithography (for the formation of initial electrodes) and the surface-catalyzed chemical deposition (for the gap narrowing), without the e-beam lithography. The PNA probes for the detection of CYP2C19 were immobilized in the gap region of INEs via Schiff base formation. The I-V characteristics clearly showed a sharp increase in the conductance between the nanogap electrodes upon the PNA-DNA hybridization, followed by the adsoprtion of functionalized Au nanoparticles. Four different target DNAs for the diagnosis of CYP2C19 polymorphism were successfully detected and discriminated with the INE-based PNA chips. © 2012 American Scientific Publishers.
- KSP Keywords
- Au nanoparticles(Au-NPs), Chemical deposition, Cytochrome P450, E-beam lithography, Effective length, GAP region, Gap distance, Genetic polymorphism, I-V characteristics, Initialization Vector(IV), PNA-DNA hybridization