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Journal Article
Microfluidic Chip Accomplishing Self-fluid Replacement using Only Capillary Force and Its Bioanalytical Application
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- Authors
- Kwang Hyo Chung, Jung Woo Hong, Dae-Sik Lee, Hyun C. Yoon
- Issue Date
- 2007-02
- Citation
- Analytica Chimica Acta, v.585, no.1, pp.1-10
- ISSN
- 0003-2670
- Publisher
- Elsevier
- Language
- English
- Type
- Journal Article
- Abstract
- A polymer microfluidic chip accomplishing automated sample flow and replacement without external controls and an application of the chip for bioanalytical reaction were described. All the fluidic operations in the chip were achieved by only natural capillary flow in a time-planned sequence. For the control of the capillary flow, the geometry of the channels and chambers in the chip was designed based on theoretical considerations and numerical simulations. The microfluidic chip was made by using polymer replication techniques, which were suitable for fast and cheap fabrication. The test for a biochemical analysis, employing an enzyme (HRP)-catalyzed precipitation reaction, exhibited a good performance using the developed chip. The presented microfluidic method would be applicable to biochemical lab-on-a-chips with integrated fluid replacement steps, such as affinity elution and solution exchange during biosensor signaling. © 2006 Elsevier B.V. All rights reserved.
- KSP Keywords
- Biochemical analysis, Capillary flow, Lab on a Chip(LoC), Microfluidic method, Numerical simulations, Precipitation reaction, Solution exchange, capillary force, microfluidic chip, polymer replication, replication techniques