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학술지 Two-Dimensional Electrophoretic Analysis of Radio Frequency Radiation-Exposed MCF7 Breast Cancer Cells
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김기범, 변해옥, 한나경, 고영규, 최형도, 김남, 백정기, 이재선
Journal of Radiation Research, v.51 no.2, pp.205-213
The Japan Radiation Research Society
10MR1300, 안전한 전자파환경 조성, 최형도
Although many in vitro studies have previously been conducted to elucidate the biological effects of radio frequency (RF) radiation over the past decades, the existence and nature of any effects is still inconclusive. In an effort to further elucidate this question, we have monitored changes in protein expression profiles in RF-exposed MCF7 human breast cancer cells using two-dimensional gel electrophoresis. MCF7 cells were exposed to 849 MHz RF radiation for 1 h per day for three consecutive days at specific absorption rates (SARs) of either 2 W/Kg or 10 W/kg. During exposure, the temperature in the exposure chamber was kept in an isothermal condition. Twenty-four hours after the final RF exposure, the protein lysates from MCF cells were prepared and two-dimensional electrophoretic analyses were conducted. The protein expression profiles of the MCF cells were not significantly altered as the result of RF exposure. None of the protein spots on the two-dimensional electrophoretic gels showed reproducible changes in three independent experiments. To determine effect of RF radiation on protein expression profiles more clearly, three spots showing altered expression without reproducibility were identified using electrospray ionization tandem mass spectrometry analysis and their expressions were examined with RT-PCR and Western blot assays. There was no alteration in their mRNA and protein levels. As we were unable to observe any significant and reproducible changes in the protein expression profiles of the RF radiation-exposed MCF7 cells using high throughput and non-high throughput techniques, it seems unlikely that RF exposure modulates the protein expression profile.
KSP 제안 키워드
1 H, Breast Cancer(BC), Electrospray ionization tandem mass spectrometry(ESI-MS/MS), Expression profiles, High throughput(HTP), Human breast cancer cells, Isothermal condition, MCF7 cells, Mass spectrometry analysis, Protein expression, Protein spots