BROWSE
Detail
Journal Article
A Temperature-Controllable Microelectrode and Its Application to Protein Immobilization
- Authors
- Dae-Sik Lee, Hyoung Gil Choi, Kwang Hyo Chung, Bun Yeoul Lee, Hyeon-Bong Pyo, Hyun C. Yoon
- Issue Date
- 2007-10
- Citation
- ETRI Journal, v.29, no.5, pp.667-669
- ISSN
- 1225-6463
- Publisher
- 한국전자통신연구원 (ETRI)
- Language
- English
- Type
- Journal Article
- Project Code
- 06MB3200, Ubiquitous Health Monitoring Module and System Development, Park Seon Hee
- Abstract
- This letter presents a smart integrated microfluidic device which can be applied to actively immobilize proteins on demand The active component in the device is a temperature-controllable microelectrode array with a smart polymer film, poly(N-isopropylacrylamide) (PNIPAAm) which can be thermally switched between hydrophilic and hydrophobic states. It is integrated into a micro hot diaphragm having an integrated micro heater and temperature sensors on a 2-micrometer-thick silicon oxide/silicon nitride/silicon oxide (O/N/O) template. Only 36 mW is required to heat the large template area of 2 mm×16 mm to 40°C within 1 second. To relay the stimulus-response activity to the microelectrode surface, the interface is modified with a smart polymer. For a model biomolecular affinity test, an anti-6-(2, 4-dinitrophenyl) aminohexanoic acid (DNP) antibody protein immobilization on the microelectrodes is demonstrated by fluorescence patterns.
- KSP Keywords
- 2 mm, Active components, Micro-heater, Microfluidic device, N-isopropylacrylamide(NIPAm), Polymer films, Silicon Nitride, Silicon oxide, Stimulus-response, Thick silicon, hydrophilic and hydrophobic