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Conference Paper
Automated Microfluidic Immunoassay (AMI) System Utilizing a Polymer Chip Equipped with a Blood Filter and Reagent Storage Chambers
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- Authors
- K.H. Chung, H.W. Song, Y.H. Choi, D.S. Lee
- Issue Date
- 2008-10
- Citation
- International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS) 2008, pp.1699-1701
- Language
- English
- Type
- Conference Paper
- Abstract
- We report an automated microfluidic immunoassay system, which consists of a highly-integrated polymer microfluidic chip, a world-to-chip interface, and a control system. We also demonstrate quantitative detection of prostate-specific antigen in the range of 1~10ng/ml using UV absorbance detection. Microfluidic components for immunoassay, such as a blood filter and reagent-storage chambers, are integrated on a planer polymer chip. The capture antibody is coated on a gold surface before introducing a sample. The microfluidic actuations for the immunoassay are accomplished sequentially and automatically without external intervention. A novel interface structure enables on-chip storage of reagents and easy handling of the chip.
- KSP Keywords
- Absorbance detection, Capture antibody, Control system, Gold surface, Interface structure, Quantitative detection, Reagent storage, UV absorbance, World-to-chip interface, microfluidic chip, on-chip