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Journal Article
Synthesis of Polystyrene Nanoparticles with Different Surface Modification by Emulsion Polymerization and Measurement of IgG Adsorption and Stability for the Application in Latex-Protein Complex Based Solid-Phase Immunoassay
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- Authors
- Sang Hee Kim, Chul Am Kim, Yo Han Choi, Moon Youn Jung
- Issue Date
- 2009-01
- Citation
- Polymer Bulletin, v.62, no.1, pp.23-32
- ISSN
- 0170-0839
- Publisher
- Springer
- Language
- English
- Type
- Journal Article
- Abstract
- Unmodified and surface modified polystyrene (PS) nanoparticles with hydrophilic end group on surface were synthesized by emulsion polymerization for adsorption of a protein (immunoglobulin G, IgG) by passive adsorption and electrostatic attraction for the application on solid-phase immunoassay. The presence of formed IgG-PS particle complex was confirmed by dynamic light scattering (DLS) measurement which shows the increased size of particle (≈178nm and 220nm) after IgG immobilization. Increased Zeta (瓘) potential from -56.7mV to -3.84mV following the IgG immobilization by electrostatic attraction also provides the presence of the formed IgG-PS particle complex. Enzyme-linked immunosorbent assay (ELISA) results demonstrate that the formed IgG-PS complex was stable and the binding strength between IgG and PS was maintained after 1 month from initial IgG-PS particle complex formation. © 2008 Springer-Verlag.
- KSP Keywords
- Complex formation, Dynamic Light Scattering, Emulsion polymerization, IgG adsorption, Immunoglobulin G, Protein complex, Sandwich enzyme-linked immunosorbent assay(ELISA), Solid phase, Surface modified polystyrene, binding strength, electrostatic attraction