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Optical Immunosensor for Quantifying C-Telopeptide Fragments of Type II Collagen as an Osteoarthritis Biomarker in Urine
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- Authors
- Su Jin Kim, Yoo Min Park, Byoung-Hyun Min, Dae-Sik Lee, Hyun C. Yoon
- Issue Date
- 2013-12
- Citation
- BioChip Journal, v.7, no.4, pp.399-407
- ISSN
- 1976-0280
- Publisher
- 한국바이오칩학회
- Language
- English
- Type
- Journal Article
- Abstract
- A fluorescence-based biosensor for measuring the quantity of C-telopeptide fragments of type II collagen (CTX-II) was developed as an osteoarthritis biomarker in urine. During osteoarthritis progression, joint components such as cartilage collagen are degraded by collagenase-protease and secreted into the serum as CTX-II. To detect CTX-II in urine (uCTX-II) having structural heterogeneity, conventional sandwich format assay does not provide sufficient accuracy. As an alternative, a competitive immunoassay was developed for the quantification of uCTX-II, which uses antibody-conjugated fluoro-microbeads to generate an optical signal. After preparing the biosensing surface, the uCTX-II sample and antibody-optical probe conjugates were reacted on the PEG4-EKGPDP exposed biosensing surface. The optical probes competed with the uCTX-II in the sample for binding to the immobilized PEG4-EKGPDP. This assay was able to detect uCTX-II concentrations between 200 ng/mmol (corrected value vs. creatinine) and 1,400 ng/mmol, encompassing the clinical detection ranges required for osteoarthritis diagnosis. The competitive immunoassay developed for uCTX-II detection was rapid and exhibited good correlation with conventional ELISA methods. This novel competition assay is a promising tool for the diagnosis and monitoring of osteoarthritis using urine samples. © 2013 The Korean BioChip Society and Springer-Verlag Berlin Heidelberg.
- KSP Keywords
- Clinical detection, Competitive immunoassay, Diagnosis and monitoring, Fluorescence-based biosensor, Fluoro-microbeads, Optical probe, Optical signal, Structural heterogeneity, Type II collagen, UCTX-II, Urine samples