상세정보
학술지
Optical Immunosensor for Quantifying C-Telopeptide Fragments of Type II Collagen as an Osteoarthritis Biomarker in Urine
- 저자
- 김수진, 박유민, 민병현, 이대식, 윤현철
- 발행일
- 201312
- 출처
- BioChip Journal, v.7 no.4, pp.399-407
- ISSN
- 1976-0280
- 출판사
- 한국바이오칩학회
- 초록
- A fluorescence-based biosensor for measuring the quantity of C-telopeptide fragments of type II collagen (CTX-II) was developed as an osteoarthritis biomarker in urine. During osteoarthritis progression, joint components such as cartilage collagen are degraded by collagenase-protease and secreted into the serum as CTX-II. To detect CTX-II in urine (uCTX-II) having structural heterogeneity, conventional sandwich format assay does not provide sufficient accuracy. As an alternative, a competitive immunoassay was developed for the quantification of uCTX-II, which uses antibody-conjugated fluoro-microbeads to generate an optical signal. After preparing the biosensing surface, the uCTX-II sample and antibody-optical probe conjugates were reacted on the PEG4-EKGPDP exposed biosensing surface. The optical probes competed with the uCTX-II in the sample for binding to the immobilized PEG4-EKGPDP. This assay was able to detect uCTX-II concentrations between 200 ng/mmol (corrected value vs. creatinine) and 1,400 ng/mmol, encompassing the clinical detection ranges required for osteoarthritis diagnosis. The competitive immunoassay developed for uCTX-II detection was rapid and exhibited good correlation with conventional ELISA methods. This novel competition assay is a promising tool for the diagnosis and monitoring of osteoarthritis using urine samples. © 2013 The Korean BioChip Society and Springer-Verlag Berlin Heidelberg.
- KSP 제안 키워드
- Clinical detection, Competitive immunoassay, Diagnosis and monitoring, Fluorescence-based biosensor, Fluoro-microbeads, Optical probe, Optical signal, Structural heterogeneity, Type II collagen, UCTX-II, Urine samples