상세정보
학술지
Optical Monitoring of Tumors in BALB/c Nude Mice Using Optical Coherence Tomography
- 발행일
- 201302
- 출처
- Journal of the Optical Society of Korea, v.17 no.1, pp.91-96
- ISSN
- 1226-4776
- 출판사
- Optical Society of America (OSA)
- 협약과제
- 12MC1600, 종양치료용 레이저 이온 가속 시스템 원천 기술 개발, 김승환
- 초록
- We report a method for optical monitoring of tumors in an animal model using optical coherence tomography (OCT). In a spectral domain OCT system, a superluminescent diode light source with a full width of 66 nm at half maximum and peak wavelength of 950 nm was used to take images having an axial resolution of 6.8 μm. Cancer cells of PC-3 were cultured and inoculated into the hypodermis of auricle tissues in BALB/c nude mice. We observed tumor formation and growth at the injection region of cancer cells in vivo and obtained the images of tumor mass center and sparse circumferences. On the 5th day from an inoculation of cancer cells, histological images of the tumor region using cross-sectional slicing and dye staining of specimens were taken in order to confirm the correlation with the high resolution OCT images. The OCT image of tumor mass compared with normal tissues was analyzed using its A-scan data so as to obtain a tissue attenuation rate which increases according to tumor growth.
- KSP 제안 키워드
- A-scan, Animal model, Attenuation rate, Axial Resolution, BALB/c nude mice, Cancer cells, Cross-sectional, Formation and growth, Full width, Half maximum, High-resolution