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Journal Article
Enhanced Protein Immobilization Efficiency on a TiO2 Surface Modified with a Hydroxyl Functional Group
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- Authors
- Wan Joong Kim, Sang Hee Kim, Bong Soo Lee, An Soon Kim, Chil Seong Ah, Chul Huh, Gun Yong Sung, Wan Soo Yun
- Issue Date
- 2009-10
- Citation
- Langmuir, v.25, no.19, pp.11692-11697
- ISSN
- 0743-7463
- Publisher
- American Chemical Society(ACS)
- Language
- English
- Type
- Journal Article
- Abstract
- An antibody immobilization was investigated using a self-assembled monolayer (SAM) over the highly refractive coatings with a SiO2, TiO2, or Si3N4 substrate. The immobilization was characterized by analyzing the hydrophilic properties of hydroxyl (OH) groups on surface coatings with contact angle (CA) measurements to enhance protein immobilization. The hydroxyl (OH) group was formed in greater amounts as the oxygen plasma exposure time was increased, which resulted in a large enhancement in antibody immobilization. It indicated that hydroxyl (OH) group formation is critical for developing a label-free optical transducer with a high sensitivity. © 2009 American Chemical Society.
- KSP Keywords
- Contact angle(CA), Exposure time, Group Formation, High Sensitivity, Hydroxyl functional group, Label-free, Optical transducer, Refractive coatings, Self-assembled monolayers(SAMS), Surface-modified, antibody immobilization