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학술대회 Undiluted Electronic Detection of Biomarkers by Si Field-Effect Transistor
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아칠성, 김안순, 김완중, 박찬우, 안창근, 양종헌, 김태엽, 성건용
International Conference on Micro and Nano Engineering (MNE) 2009, pp.1-2
09IC1500, 유비쿼터스 건강관리용 모듈 시스템, 김승환
In this study, we have developed a new detection method using Si field effect transistor (FET)-type biosensors, which enables the direct monitoring of antigen-antibody binding within very high-ionic-strength solutions such as 1×PBS and human serum. In the new method, as no additional dilution or desalting processes are required, the FET-type biosensors can be more suitable for ultrasensitive and real-time analysis of raw sample solutions. The new detection scheme is based on the observation that the strength of antigen-antibody-specific binding is significantly influenced by the ionic strength of the reaction solutions. For a prostate specific antigen (PSA), in some conditions, the binding reaction between PSA and anti-PSA in a low-ionic strength reaction solution such as 10 μM phosphate buffer is weak (reversible), while that in high-ionic strength reaction solutions such as 1×PBS or human serum is strong.
KSP 제안 키워드
Antigen-antibody binding, Binding reaction, Detection Method, Detection scheme, Electronic detection, Field-effect transistors(FETs), Human serum, Ionic strength, Phosphate buffer, Real-time Analysis, Si field-effect transistor