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학술대회 Analysis of Configuration of Surface-Immobilized Proteins by Si Nanochannel Field Effect Transistor Biosensor
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박찬우, 아칠성, 안창근, 양종헌, 김안순, 김태엽, 성건용
Eurosensors 2009, pp.674-677
08MC2500, 유비쿼터스 건강관리용 모듈 시스템, 박선희
We demonstrate the application of 'top-down' fabricated Si nanochannel field effect transistor biosensors in measuring the configuration of charged proteins immobilized on the Si surface. We have immobilized monoclonal antibodies of PSA (anti-PSA) on the p-type Si channel surface, and monitored the channel conductance in response to the variation of ionic strength (salt concentration) and pH level of the buffer solution. The dependency of channel conductance on the salt concentration is consistent with the screening of protein charges by dissolved counterions, enabling quantitative estimation of the distance from the Si surface to anti-PSA molecules based on calculated Debye screening lengths ({\\lambda}D) for each salt concentration. The effect of salt concentration on the pH-dependency of channel conductance has also been studied, and explained in terms of the net charge of anti-PSA as a function of pH. © 2009.
KSP 제안 키워드
Buffer solution, Debye screening, Field effect transistor biosensor, Field-effect transistors(FETs), Ionic strength, Monoclonal antibody(MAb), PH level, Quantitative estimation, Salt concentration, Si channel, Si nanochannel