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Conference Paper Analysis of Configuration of Surface-Immobilized Proteins by Si Nanochannel Field Effect Transistor Biosensor
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Authors
Park Chan Woo, Chil Seong Ah, Chang-Geun Ahn, Yang Jong-Heon, Kim Ansoon, Tae-Youb Kim, Gun Yong Sung
Issue Date
200909
Source
Eurosensors 2009, pp.674-677
DOI
https://dx.doi.org/10.1016/j.proche.2009.07.168
Project Code
08MC2500, Ubiquitous Health Monitoring Module and System Development, Park Seon Hee
Abstract
We demonstrate the application of 'top-down' fabricated Si nanochannel field effect transistor biosensors in measuring the configuration of charged proteins immobilized on the Si surface. We have immobilized monoclonal antibodies of PSA (anti-PSA) on the p-type Si channel surface, and monitored the channel conductance in response to the variation of ionic strength (salt concentration) and pH level of the buffer solution. The dependency of channel conductance on the salt concentration is consistent with the screening of protein charges by dissolved counterions, enabling quantitative estimation of the distance from the Si surface to anti-PSA molecules based on calculated Debye screening lengths ({\\lambda}D) for each salt concentration. The effect of salt concentration on the pH-dependency of channel conductance has also been studied, and explained in terms of the net charge of anti-PSA as a function of pH. © 2009.
KSP Keywords
Buffer solution, Debye screening, Field effect transistor biosensor, Field-effect transistors(FETs), Ionic strength, Monoclonal antibody(MAb), PH level, Quantitative estimation, Salt concentration, Si channel, Si nanochannel