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학술지 Synthesis of Anionic Sulfate Functionalized Polystyrene Latex and Protein Adsorption for Application as a Biosensor
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김상희, 김철암, 표현봉, 정문연
Journal of Nanoscience and Nanotechnology, v.9 no.12, pp.7171-7176
American Scientific Publishers (ASP)
Bare and surface modified polystyrene nanoparticles with an anionic sulfate end group were synthesized by emulsion polymerization for adsorption of a protein (immunoglobulin G, IgG) by passive adsorption and electrostatic attraction for application as a biosensor. Dynamic light scattering (DLS) measurements confirmed the formation of an IgG-polystyrene latex complex, showing increased hydrodynamic diameter (D h) of ≈178 nm (passive adsorption) and ≈220 nm (electrostatic attraction) after IgG adsorption. An increased Zeta (??) potential value from -38.8 mV to -3.84 mV following IgG adsorption by electrostatic attraction also reflected the formation of the IgG-polystyrene latex complex. Solid phase immunodetection results demonstrated the formation of the IgG-polystyrene latex complex by showing fluorescent signals induced by FITC, which conjugated to IgG on the surface of the polystyrene. In addition, immunoassay results showed that adsorbed IgG on the polystyrene latex surface was not desorbed and maintained immunoreactivity after 1 month from the initial IgG-polystyrene latex complex formation. © 2009 American Scientific Publishers. All rights reserved.
KSP 제안 키워드
20 nm, Complex formation, Dynamic Light Scattering, Emulsion polymerization, End group, Functionalized polystyrene, IgG adsorption, Immunoglobulin g(IGG), Protein Adsorption, Solid phase, Surface modified polystyrene