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Magnetic Immunoassay Platform based on the Planar Frequency Mixing Magnetic Technique
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- Authors
- Chang-Beom Kim, Eul-Gyoon Lim, Sung Woong Shin, Hans Joachim Krause, Hyobong Hong
- Issue Date
- 2016-09
- Citation
- Biosensors and Bioelectronics, v.83, pp.293-299
- ISSN
- 0956-5663
- Publisher
- Elsevier
- Language
- English
- Type
- Journal Article
- Abstract
- We represent the experimental results of our planar-frequency mixing magnetic detection (p-FMMD) technique to obtain 2D superparamagnetic images for magnetic immunoassay purpose. The imaging of magnetic beads is based on the nonlinear magnetic characteristics inherent in superparamagnetic materials. The p-FMMD records the sum-frequency components originating from both a high and a low frequency magnetic field incident on the magnetically nonlinear nanoparticles. In this study, we apply the p-FMMD technique to 2D scanning imaging of superparamagnetic iron oxide nanoparticles (SPIONs) in a microfluidic platform. Our p-FMMD system enables to acquire planar images of SPIONs filled in a microchannel as narrow as 30 μm in width. The minimum detectable amount is ~1.0×108 beads of 100 nm size. The system shows a spatial resolution enabling to distinguish between two distinct channels even 2 mm apart from each other. Our p-FMMD system as a magnetic immunoassaying system has permitted the detection of amyloid beta 42 (A棺42), a promising biomarker of Alzheimer's disease, at the minimum concentration of 23.8 pg/ml. This may enable the identification of the A棺42 levels for the early-stage of Alzheimer's disease with the assistance of the MPI using p-FMMD technique. The results show that the deployment of the p-FMMD can be an alternative to conventional biosensing analytical methods, and can be used as a fast and portable screening method.
- KSP Keywords
- 2 mm, 2D scanning, Alzheimer's Disease(AD), Amyloid beta, Analytical Method, Early stages, Frequency components, Low Frequency Magnetic Field, Magnetic beads(MBs), Magnetic characteristics, Magnetic field(MF)