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Detail
Journal Article
Synergistic Use of Gold Nanoparticles (AuNPs) and “Capillary Enzyme-Linked Immunosorbent Assay (ELISA)” for High Sensitivity and Fast Assays
- Authors
- Wan-Joong Kim, Hyo Young Cho, Bongjin Jeong, Sangwon Byun, JaeDoo Huh, Young Jun Kim
- Issue Date
- 2018-01
- Citation
- Sensors, v.18, no.1, pp.1-12
- ISSN
- 1424-8220
- Publisher
- MDPI AG
- Language
- English
- Type
- Journal Article
- Abstract
- Using gold nanoparticles (AuNPs) on ?쐁apillary enzyme-linked immunosorbent assay (ELISA)", we produced highly sensitive and rapid assays, which are the major attributes for point-of-care applications. First, in order to understand the size effect of AuNPs, AuNPs of varying diameters (5 nm, 10 nm, 15 nm, 20 nm, 30 nm, and 50 nm) conjugated with Horseradish Peroxidase (HRP)-labeled anti-C reactive protein (antiCRP) (AuNP?줭ntiCRP-HRP) were used for well-plate ELISA. AuNP of 10 nm produced the largest optical density, enabling detection of 0.1 ng/mL of CRP with only 30 s of incubation, in contrast to 10 ng/mL for the ELISA run in the absence of AuNP. Then, AuNP of 10 nm conjugated with antiCRP-HRP (AuNP?줭ntiCRP-HRP) was used for ?쐁apillary ELISA?? to detect as low as 0.1 ng/mL of CRP. Also, kinetic study on both 96-well plates and in a capillary tube using antiCRP-HRP or AuNP?줭ntiCRP-HRP showed a synergistic effect between AuNP and the capillary system, in which the fastest assay was observed from the ?쏛uNP capillary ELISA??, with its maximum absorbance reaching 2.5 min, while the slowest was the typical well-plate ELISA with its maximum absorbance reaching in 13.5 min.
- KSP Keywords
- 20 nm, 5 nm, 96-well, C-reactive protein(CRP), Capillary system, Capillary tube, High Sensitivity, Highly sensitive, Kinetic study, Optical density, Point-of-care
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(CC BY)
