BROWSE
Detail
Journal Article
Layer-by-layer Electrochemical Biosensors Configuring Xanthine Oxidase and Carbon Nanotubes/graphene Complexes for Hypoxanthine and Uric Acid in Human Serum Solutions
Cited 58 time in 
Share 
Share
- Authors
- Yunpei Si, Jeong Won Park, Sunhee Jung, Geum-Sook Hwang, Eunseo Goh, Hye Jin Lee
- Issue Date
- 2018-12
- Citation
- Biosensors and Bioelectronics, v.121, pp.265-271
- ISSN
- 0956-5663
- Publisher
- Elsevier
- Language
- English
- Type
- Journal Article
- Abstract
- A selective biosensing platform for the determination of hypoxanthine (Hx) and uric acid (UA) concentrations in both buffer and human serum sample solutions was developed. The biosensor features the layer-by-layer (LbL) self-assembly of negatively charged xanthine oxidase (XOD) and positively charged poly(diallyldimethyl ammonium chloride) (PDDA) wrapped oxidized multi-walled carbon nanotubes and graphene (CNTs-G) complexes (PDDA-CNTs-G) on screen printed carbon electrode (SPCE) surfaces. Catalytic responses of the XOD modified biosensor with the chosen optimum number of layers for LbL assembly on SPCE towards Hx in buffer solutions were first investigated using both cyclic and square wave voltammetries. The peak current at around 0.08 V (vs. Ag/AgCl) associated with the production of UA increased as a function of the Hx concentration due to the surface selective catalytic reaction of XOD and Hx. A linear dynamic range of 5??50 쨉M Hx with a detection limit of 4.40 쨉M was obtained and the sensor was further applied to the analysis of Hx in normal human serum solutions in addition to myocardial infarction (MI) patient serum sample solutions from a local hospital. Since untreated serum solutions contain a certain amount of UA, a XOD free SPCE biosensor consisted of only PDDA-CNTs-G was also employed to evaluate the native concentration of UA in the serum and further assist the determination of Hx concentration when using the developed LbL biosensor. Our sensing results for the real biological fluidic solutions were finally validated by comparing to those using liquid chromatography-mass spectrometry(LC-MS).
- KSP Keywords
- Biosensing platform, Carbon nano-tube(CNT), Cyclic and square wave voltammetries, Detection limit, Electrochemical biosensors, Human serum sample, LBL assembly, Linear dynamic range, Liquid chromatography(LC), Liquid chromatography-mass spectrometry(LC-MS), Myocardial Infarction(MI)