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Journal Article Multichannel Surface Plasmon Resonance Imaging and Analysis of Micropatterned Self-Assembled Monolayers and Protein Affinity Interactions
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Authors
Hyeon-Bong Pyo, 신용범, 김민곤, 윤현철
Issue Date
200501
Source
Langmuir, v.21 no.1, pp.166-171
ISSN
0743-7463
Publisher
American Chemical Society (ACS)
DOI
https://dx.doi.org/10.1021/la0486382
Abstract
Multichannel images of 11-mercaptoundecanoic acid and 11-mercapto-1-undecanol self-assembled monolayers together with a biospecific interferon-款 (IFN-款)/anti-IFN-款 antibody immunoaffinity interaction were observed by the two-dimensional surface plasmon resonance (2D-SPR) imaging system. With the fabricated 2D-SPR imaging system, adopting a white light source in combination with a narrow band-pass filter, sharp images were resolved, minimizing the diffraction patterns on the resulting images. Micropatterning of self-assembled monolayers was acheived by exploiting the UV photolysis of thiol bonding, instead of conventional photolithography. The line profile calibration of the image contrast with ellipsometric analysis enabled us to discriminate the change in monolayer thickness within a sub-nanometer scale. For the protein interactions on the surface, the biospecific affinity recognition reaction of IFN-款 antigen with surface-immobilized antibody was analyzed. Through the signal amplification strategy based on the enzymecatalyzed precipitation reaction in a sandwich-type immunoassay, biospecific antigen binding was found detectable down to a concentration of 1 ng/mL.
KSP Keywords
1-Undecanol, 11-mercaptoundecanoic acid, Affinity interactions, Band-pass filter(BPF), Biospecific affinity, Image Contrast, Immobilized antibodies, Line profile, Nanometer-scale, Narrow band-pass filter, Precipitation reaction