상세정보
학술지
Multichannel Surface Plasmon Resonance Imaging and Analysis of Micropatterned Self-Assembled Monolayers and Protein Affinity Interactions
- 저자
- 표현봉, 신용범, 김민곤, 윤현철
- 발행일
- 200501
- 출처
- Langmuir, v.21 no.1, pp.166-171
- ISSN
- 0743-7463
- 출판사
- American Chemical Society (ACS)
- 초록
- Multichannel images of 11-mercaptoundecanoic acid and 11-mercapto-1-undecanol self-assembled monolayers together with a biospecific interferon-款 (IFN-款)/anti-IFN-款 antibody immunoaffinity interaction were observed by the two-dimensional surface plasmon resonance (2D-SPR) imaging system. With the fabricated 2D-SPR imaging system, adopting a white light source in combination with a narrow band-pass filter, sharp images were resolved, minimizing the diffraction patterns on the resulting images. Micropatterning of self-assembled monolayers was acheived by exploiting the UV photolysis of thiol bonding, instead of conventional photolithography. The line profile calibration of the image contrast with ellipsometric analysis enabled us to discriminate the change in monolayer thickness within a sub-nanometer scale. For the protein interactions on the surface, the biospecific affinity recognition reaction of IFN-款 antigen with surface-immobilized antibody was analyzed. Through the signal amplification strategy based on the enzymecatalyzed precipitation reaction in a sandwich-type immunoassay, biospecific antigen binding was found detectable down to a concentration of 1 ng/mL.
- KSP 제안 키워드
- 1-Undecanol, 11-mercaptoundecanoic acid, Affinity interactions, Band-pass filter(BPF), Biospecific affinity, Image Contrast, Immobilized antibodies, Line profile, Nanometer-scale, Narrow band-pass filter, Precipitation reaction