상세정보
학술지
Activation of Matrix Metalloproteinases and FoxO3a in HaCaT Keratinocytes by Radiofrequency Electromagnetic Field Exposure
- 발행일
- 202104
- 출처
- Scientific Reports, v.11, pp.1-10
- ISSN
- 2045-2322
- 출판사
- Nature Research
- 협약과제
- 21HH2400, 복합 전파환경에서의 국민건강 보호 기반 구축 사업, 최형도
- 초록
- As the skin is the largest body organ and critically serves as a barrier, it is frequently exposed and could be physiologically affected by radiofrequency electromagnetic field (RF-EMF) exposure. In this study, we found that 1760혻MHz RF-EMF (4.0혻W/kg specific absorption rate for 2혻h/day during 4 days) exposure could induce intracellular reactive oxygen species (ROS) production in HaCaT human keratinocytes using 2??,7??-dichlorofluorescin diacetate fluorescent probe analysis. However, cell growth and viability were unaffected by RF-EMF exposure. Since oxidative stress in the skin greatly influences the skin-aging process, we analyzed the skin senescence-related factors activated by ROS generation. Matrix metalloproteinases 1, 3, and 7 (MMP1, MMP3, and MMP7), the main skin wrinkle-related proteins, were significantly increased in HaCaT cells after RF-EMF exposure. Additionally, the gelatinolytic activities of secreted MMP2 and MMP9 were also increased by RF-EMF exposure. FoxO3a (Ser318/321) and ERK1/2 (Thr 202/Tyr 204) phosphorylation levels were significantly increased by RF-EMF exposure. However, Bcl2 and Bax expression levels were not significantly changed, indicating that the apoptotic pathway was not activated in keratinocytes following RF-EMF exposure. In summary, our findings show that exposure to 1760혻MHz RF-EMF induces ROS generation, leading to MMP activation and FoxO3a and ERK1/2 phosphorylation. These data suggest that RF-EMF exposure induces cellular senescence of skin cells through ROS induction in HaCaT human keratinocytes.
- KSP 제안 키워드
- Aging process, Cell growth, EMF exposure, Electromagnetic Field Exposure, HaCaT cells, Human keratinocytes, Matrix metalloproteinases, Oxidative Stress, ROS generation, Reactive oxygen species (ros), Skin cells
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