ETRI-Knowledge Sharing Plaform



논문 검색
구분 SCI
연도 ~ 키워드


학술지 Novel Electrochemical PMI Marker Biosensor Based on Quantum Dot Dissolution Using a Double-label Strategy
Cited 3 time in scopus Download 72 time Share share facebook twitter linkedin kakaostory
정봉진, Rashida Akter, 오정현, 이동기, 안창근, 최종순, Md. Aminur Rahman
Scientific Reports, v.12, pp.1-8
Nature Research
21HR3800, Sub-ppb급 가스성분 감지를 위한 후각지능 기술 개발, 김승환
A novel and facile post-mortem interval (PMI) biosensor was fabricated using a double-label strategy to detect the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) biomarker. A monoclonal anti-GAPDH antibody was immobilized on a surface label containing cadmium selenide quantum dots (CdSe QDs) on a cysteamine graphene oxide (Cys-GO) self-assembled monolayer. Glucose oxidase (GOx) was used as a signal label to conjugate with GAPDH. GAPDH recognition was achieved through the dissolution of the surface-attached CdSe QDs by hydrogen peroxide generated through GAPDH-conjugated GOx-catalyzed 棺-glucose oxidation. To enhance sensitivity, a competitive interaction was introduced between free and conjugated GAPDH to the active site of the anti-GAPDH antibody. The electrochemical response due to CdSe dissolution decreased proportionally with the concentration of free GAPDH. Differential pulsed voltammetry was conducted to determine the analytical characteristics of the immunosensor, including the limit of detection, linear dynamic range, target selectivity, system stability, and applicability toward the analysis of real samples.
KSP 제안 키워드
Active sites, Cadmium selenide quantum dots, CdSe QDs, Electrochemical response, Glucose oxidase(GOD), Glyceraldehyde 3-phosphate dehydrogenase, Graphene oxide(GOS), Limit of detection, Linear dynamic range, Post-mortem, Pulsed voltammetry
본 저작물은 크리에이티브 커먼즈 저작자 표시 (CC BY) 조건에 따라 이용할 수 있습니다.
저작자 표시 (CC BY)