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Journal Article Novel Electrochemical PMI Marker Biosensor Based on Quantum Dot Dissolution Using a Double-label Strategy
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Authors
Bongjin Jeong, Rashida Akter, Jeonghyun Oh, Dong-Gi Lee, Chang-Geun Ahn, Jong-Soon Choi, Md. Aminur Rahman
Issue Date
2022-05
Citation
Scientific Reports, v.12, pp.1-8
ISSN
2045-2322
Publisher
Nature Research
Language
English
Type
Journal Article
DOI
https://dx.doi.org/10.1038/s41598-022-12444-6
Abstract
A novel and facile post-mortem interval (PMI) biosensor was fabricated using a double-label strategy to detect the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) biomarker. A monoclonal anti-GAPDH antibody was immobilized on a surface label containing cadmium selenide quantum dots (CdSe QDs) on a cysteamine graphene oxide (Cys-GO) self-assembled monolayer. Glucose oxidase (GOx) was used as a signal label to conjugate with GAPDH. GAPDH recognition was achieved through the dissolution of the surface-attached CdSe QDs by hydrogen peroxide generated through GAPDH-conjugated GOx-catalyzed 棺-glucose oxidation. To enhance sensitivity, a competitive interaction was introduced between free and conjugated GAPDH to the active site of the anti-GAPDH antibody. The electrochemical response due to CdSe dissolution decreased proportionally with the concentration of free GAPDH. Differential pulsed voltammetry was conducted to determine the analytical characteristics of the immunosensor, including the limit of detection, linear dynamic range, target selectivity, system stability, and applicability toward the analysis of real samples.
KSP Keywords
Active sites, Cadmium selenide quantum dots, CdSe QDs, Electrochemical response, Glucose oxidase(GOD), Glyceraldehyde 3-phosphate dehydrogenase, Graphene oxide(GOS), Limit of detection, Linear dynamic range, Post-mortem, Pulsed voltammetry
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