상세정보
학술지
Novel Electrochemical PMI Marker Biosensor Based on Quantum Dot Dissolution Using a Double-label Strategy
- 발행일
- 202205
- 출처
- Scientific Reports, v.12, pp.1-8
- ISSN
- 2045-2322
- 출판사
- Nature Research
- 협약과제
- 21HR3800, Sub-ppb급 가스성분 감지를 위한 후각지능 기술 개발, 김승환
- 초록
- A novel and facile post-mortem interval (PMI) biosensor was fabricated using a double-label strategy to detect the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) biomarker. A monoclonal anti-GAPDH antibody was immobilized on a surface label containing cadmium selenide quantum dots (CdSe QDs) on a cysteamine graphene oxide (Cys-GO) self-assembled monolayer. Glucose oxidase (GOx) was used as a signal label to conjugate with GAPDH. GAPDH recognition was achieved through the dissolution of the surface-attached CdSe QDs by hydrogen peroxide generated through GAPDH-conjugated GOx-catalyzed 棺-glucose oxidation. To enhance sensitivity, a competitive interaction was introduced between free and conjugated GAPDH to the active site of the anti-GAPDH antibody. The electrochemical response due to CdSe dissolution decreased proportionally with the concentration of free GAPDH. Differential pulsed voltammetry was conducted to determine the analytical characteristics of the immunosensor, including the limit of detection, linear dynamic range, target selectivity, system stability, and applicability toward the analysis of real samples.
- KSP 제안 키워드
- Active sites, Cadmium selenide quantum dots, CdSe QDs, Electrochemical response, Glucose oxidase(GOD), Glyceraldehyde 3-phosphate dehydrogenase, Graphene oxide(GOS), Limit of detection, Linear dynamic range, Post-mortem, Pulsed voltammetry
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