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Journal Article
Rapid Autofocusing Via 1 Bit Transform for Real-Time System to Detect Mycobacterium Tuberculosis
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- Authors
- C. Jung
- Issue Date
- 2014-02
- Citation
- Electronics Letters, v.50, no.5, pp.348-349
- ISSN
- 0013-5194
- Publisher
- IET
- Language
- English
- Type
- Journal Article
- Abstract
- Autofocusing is a key building block in the modern automated highthroughput systems for a quantitative analysis of microscopy images. For that reason, it has received great attention, and diverse autofocus algorithms have been proposed in the literature. A fast autofocus algorithm is presented in order to build a real-time system for diagnosing the sputum smear samples. The method involves transforming the sputum smear fluorescence images from a multibit to a 1 bit/pixel representation. This results in a substantial reduction in the computational cost, while maintaining a good focusing performance. Experimental results are presented to validate the feasibility and the effectiveness of the proposed method. © 2014 The Institution of Engineering and Technology.
- KSP Keywords
- Focusing performance, Mycobacterium tuberculosis(MTB), Quantitative analysis, Real-Time Systems, building block, computational cost, microscopy images