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Conference Paper
Fast Pcr Utilizing Buoyant Convection in a Disposable Chip
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- Authors
- K.H. Chung, Y.H. Choi, B.K. Kim, G.Y. Sung
- Issue Date
- 2011-01
- Citation
- International Conference on Micro Electro Mechanical Systems (MEMS) 2011, pp.865-868
- Language
- English
- Type
- Conference Paper
- Abstract
- We report a portable polymerase chain reaction(PCR) device conducting multiple PCR reactions in less than 5 minutes. We designed a novel loop-shaped microchannel in order to circulate PCR reactants fast through it. Temperature differences among three zones in the microchannel continuously generate a buoyant convection along the microchannel. The microchannel is molded in a disposable polymer chip. Our group's first prototype was reported previously, and we present a newly optimized device of the enhanced performance especially in PCR speed and multiplexing. The buoyant flows in the microchannel are newly visualized and compared with results from numerical simulations.
- KSP Keywords
- Buoyant convection, Enhanced performance, Numerical simulation(Trnsys16), Polymerase chain reaction, Shaped microchannel, fast PCR, temperature difference