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Registered NATURAL CONVECTION-DRIVEN PCR APPARATUS AND METHOD USING DISPOSABLE POLYMER CHIP

일회용 폴리머 칩을 이용한 자연대류 PCR 방법과 장치
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Inventors
Chung Kwang Hyo, Hyeon-Bong Pyo, Park Seon Hee, Dae-Sik Lee
Application No.
11866979 (2007.10.03)
Publication No.
20080131956 (2008.06.05)
Registration No.
8735103 (2014.05.27)
Country
UNITED STATES
Abstract
Provided is a natural convection-driven polymerase chain reaction (PCR) apparatus and method, which can periodically change temperature of a sample to make a natural convection-driven flow in a loop-shaped channel on a disposable polymer chip and contacting heating metal members maintained at different temperatures with channels. The natural convection-driven PCR apparatus includes: a polymer chip having a plurality of channels connected together to form a loop-shaped microchannel, a sample being filled inside the channels; a plurality of heating metal members contacting the loop-shaped microchannel of the polymer chip and transferring heat; a temperature controller connected to the heating metal members to maintain the heating metal members at different temperatures; a plurality of heaters connected to the heating metal members and the temperature controller to supply heat to the heating metal members; and temperature sensors connected to the heating metal members and the temperature controller.
KSP Keywords
Different temperatures, Natural Convection, Polymerase chain reaction, Shaped microchannel, Temperature controller, chain reaction, temperature sensor(LM35)